產(chǎn)品介紹

Biocolor 彈性蛋白檢測(cè)試劑盒
Biocolor Fastin elastin Assay 

利用染料結(jié)合的方法來定量檢測(cè)釋放到組織培養(yǎng)液或從生物材料中提取的彈性蛋白。
 
應(yīng)用范圍：樣本可來源于細(xì)胞培養(yǎng)基和含細(xì)胞外基質(zhì)成分的組織提取物（如彈性軟骨、主動(dòng)脈、皮膚等），可檢測(cè)可溶性彈性蛋白原和不可溶性彈性蛋白（抽提后以可溶性彈性蛋白多肽的形式檢測(cè)）。
 
樣本要求：樣本體積5-100ul，包含5-75ug的彈性蛋白
 
實(shí)驗(yàn)操作步驟

彈性蛋白的分離：用彈性蛋白沉淀劑處理樣本，使彈性蛋白沉淀下來。
彈性蛋白與染料結(jié)合：裝有沉淀的彈性蛋白管中，加染料和反應(yīng)液，反應(yīng)60min以上
彈性蛋白-染料復(fù)合物獲得：去掉未結(jié)合的染料
復(fù)合物染料的釋放：添加染料釋放劑，使彈性蛋白-染料復(fù)合物中的染料釋放出來
釋放出的染料檢測(cè)：繪制標(biāo)準(zhǔn)曲線，513nm或405nm處定量檢測(cè)

產(chǎn)品訂購

   Product Code: F2000        110 assays

   Product Code: F4000        440 assays

參考文獻(xiàn)

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